5UHQ

Structure of a SemiSWEET Q20A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0333
Spacegroup name	C 2 2 21
Unit cell lengths	63.709, 189.014, 90.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 2.780
R-factor	0.2313
Rwork	0.227
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qnc
RMSD bond length	0.005
RMSD bond angle	1.205
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.760
High resolution limit [Å]	2.780
Number of reflections	13946
<I/σ(I)>	10.9
Completeness [%]	98.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	9.7 MAG 50 mM sodium acetate pH 4.5 100 mM ZnCl2 16% PEG 400