5UHP
Crystal structure of the core catalytic domain of human O-GlcNAcase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 89.722, 91.531, 94.517 |
Unit cell angles | 77.27, 62.81, 63.03 |
Refinement procedure
Resolution | 40.780 - 2.790 |
R-factor | 0.242 |
Rwork | 0.239 |
R-free | 0.29200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.780 |
High resolution limit [Å] | 2.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M K Na tartrate tetrahydrate, 20% PEG 3350 |