5UHB
Crystal structure of Mycobacterium tuberculosis transcription initiation complex in complex with Rifampin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.927 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 154.190, 164.234, 200.203 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.782 - 4.290 |
R-factor | 0.2122 |
Rwork | 0.208 |
R-free | 0.26670 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.400 |
High resolution limit [Å] | 4.250 | 9.140 | 4.250 |
Rmerge | 0.066 | 0.029 | 0.543 |
Rmeas | 0.072 | 0.032 | 0.609 |
Rpim | 0.028 | 0.012 | 0.270 |
Total number of observations | 198467 | ||
Number of reflections | 33869 | ||
<I/σ(I)> | 8.5 | ||
Completeness [%] | 97.0 | 98.2 | 89 |
Redundancy | 5.9 | 6.5 | 4.5 |
CC(1/2) | 0.996 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 50 mM Tris-HCl, pH 8.2, 200 mM KCl, 50 mM MgCl2, and 10% PEG3350 |