5UH8
Crystal structure of Mycobacterium tuberculosis transcription initiation complex containing 4nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-09 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 1.03316 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 151.995, 163.666, 197.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.640 - 4.176 |
R-factor | 0.2045 |
Rwork | 0.201 |
R-free | 0.25690 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.350 |
High resolution limit [Å] | 4.200 | 9.030 | 4.200 |
Rmerge | 0.172 | 0.106 | 0.669 |
Rmeas | 0.183 | 0.112 | 0.740 |
Rpim | 0.060 | 0.036 | 0.299 |
Total number of observations | 280372 | ||
Number of reflections | 35874 | ||
<I/σ(I)> | 6.6 | ||
Completeness [%] | 96.1 | 99.6 | 82.6 |
Redundancy | 7.8 | 9.7 | 4.4 |
CC(1/2) | 0.974 | 0.489 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 50 mM Tris-HCl, pH 8.2, 200 mM KCl, 50 mM MgCl2, and 10% PEG3350 |