5UGQ
Crystal Structure of Hip1 (Rv2224c)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.260, 104.260, 127.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.492 - 2.609 |
| R-factor | 0.2066 |
| Rwork | 0.203 |
| R-free | 0.24270 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.185 | 0.083 | 0.944 |
| Rmeas | 0.189 | ||
| Rpim | 0.041 | ||
| Number of reflections | 24929 | ||
| <I/σ(I)> | 4.9 | ||
| Completeness [%] | 99.3 | 99.7 | 93.8 |
| Redundancy | 20.7 | 20.2 | 16.3 |
| CC(1/2) | 0.999 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% (W/V) PEG3350, 0.1 M NaAcetate pH 5.6, 0.2 M (NH4)2SO4, 10% (V/V) glycerol |
