5UGO
DNA polymerase beta nick complex with imidodiphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-02 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.596, 79.312, 55.194 |
| Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
| Resolution | 23.811 - 1.897 |
| R-factor | 0.1822 |
| Rwork | 0.179 |
| R-free | 0.23560 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.811 | 1.970 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.073 | 0.450 |
| Number of reflections | 32852 | |
| <I/σ(I)> | 19.8 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 7.5 |
