5UGN
DNA polymerase beta imidodiphosphate reactant complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-07 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.778, 79.922, 55.321 |
| Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
| Resolution | 23.154 - 1.997 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.154 | 2.030 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Rmerge | 0.074 | 0.504 |
| Number of reflections | 28404 | 1317 |
| <I/σ(I)> | 20.2 | 2.2 |
| Completeness [%] | 99.2 | 92.4 |
| Redundancy | 5.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350 |
