5UFL
Crystal structure of a CIP2A core domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 65 |
| Unit cell lengths | 153.276, 153.276, 105.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 132.740 - 3.000 |
| R-factor | 0.2569 |
| Rwork | 0.256 |
| R-free | 0.28120 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 132.740 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 28364 |
| <I/σ(I)> | 25.4 |
| Completeness [%] | 99.9 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Sodium malonate pH6.0, 7% PEG 4000, 1% PEG8000 |
