5UFK
Structure of the effector protein SidK (lpg0968) from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-14 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.7712 |
Spacegroup name | P 43 |
Unit cell lengths | 52.960, 52.960, 127.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.905 - 2.300 |
R-factor | 0.1744 |
Rwork | 0.172 |
R-free | 0.21230 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.496 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.096 | 1.060 |
Number of reflections | 30728 | |
<I/σ(I)> | 16.8 | 2.18 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 7.6 | 7.4 |
CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TRIS hydrochloride pH 8.5, 0.2 M lithium sulfate monohydrate and 30% (w/v) polyethylene glycol 4,000 |