5UFF
Crystal Structure of Variable Lymphocyte Receptor (VLR) RBC36 with Fucose(alpha-1-2)Lactose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97840 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.969, 47.503, 57.276 |
Unit cell angles | 90.00, 85.69, 90.00 |
Refinement procedure
Resolution | 39.856 - 2.137 |
R-factor | 0.2259 |
Rwork | 0.223 |
R-free | 0.28240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e6j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.117 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.865 | 2.210 |
High resolution limit [Å] | 2.137 | 2.137 |
Rpim | 0.070 | 0.700 |
Number of reflections | 11758 | |
<I/σ(I)> | 8 | |
Completeness [%] | 97.0 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 11.4 mg/mL RBC36, 6.9 mM fucose(alpha-1-2)lactose, 140 mM ammonium chloride, 22% PEG3350 |