5UF4
Crystal Structure of Variable Lymphocyte Receptor (VLR) O13 with LNnT bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 82.423, 82.423, 227.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.809 - 2.040 |
R-factor | 0.1959 |
Rwork | 0.195 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uei |
RMSD bond length | 0.004 |
RMSD bond angle | 0.756 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.810 | 46.810 | 2.080 |
High resolution limit [Å] | 2.040 | 5.530 | 2.040 |
Rmerge | 0.220 | 0.101 | 0.618 |
Number of reflections | 46172 | ||
<I/σ(I)> | 6.4 | ||
Completeness [%] | 90.4 | 100 | 44.4 |
Redundancy | 7.2 | 8.9 | 2 |
CC(1/2) | 0.996 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 7.5 mg/mL O13 protein, 1.25 mM LnNT, 20% Peg4000, 0.2 M ammonium sufate, 0.1 M sodium acetate, pH 5.0 |