5UEY
BRD4_BD2_A-1412838
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-02 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.185, 74.627, 33.376 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.380 - 2.410 |
R-factor | 0.272 |
Rwork | 0.270 |
R-free | 0.31000 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.400 |
High resolution limit [Å] | 2.410 |
Number of reflections | 5571 |
<I/σ(I)> | 18.9 |
Completeness [%] | 99.7 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0 |