5UEW
BRD2 Bromodomain2 with A-1360579
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 35.996, 41.601, 45.756 |
Unit cell angles | 67.40, 66.90, 74.85 |
Refinement procedure
Resolution | 18.270 - 1.830 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23800 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS (2.11.7) |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.900 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.056 |
Number of reflections | 18605 |
<I/σ(I)> | 6.5 |
Completeness [%] | 94.8 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | Protein buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0 |