5UEL
Crystal structure of 354NC102 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.218, 82.499, 117.211 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.910 - 1.600 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.20130 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.145 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.910 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.108 |
Number of reflections | 77546 |
<I/σ(I)> | 11.12 |
Completeness [%] | 100.0 |
Redundancy | 6.7 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate trihydrate pH 4.6, 2.0 M ammonium sulfate |