5UEK

Structure of antigen-Fab 12E complex with Histone chaperone ASF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	80
Detector technology	CCD
Collection date	2014-02-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	43.175, 142.932, 49.401
Unit cell angles	90.00, 98.80, 90.00

Refinement procedure

Resolution	22.458 - 1.700
R-factor	0.1782
Rwork	0.176
R-free	0.21250
RMSD bond length	0.006
RMSD bond angle	0.832
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	22.460
High resolution limit [Å]	1.700
Number of reflections	64692
<I/σ(I)>	22.1
Completeness [%]	81.4
Redundancy	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 20% ethylene glycol, .2 M Ammonium Acetate