5UEI

Crystal Structure of Variable Lymphocyte Receptor (VLR) O13 (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41
Unit cell lengths	86.663, 86.663, 38.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.331 - 1.702
R-factor	0.1598
Rwork	0.159
R-free	0.18460
RMSD bond length	0.012
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.086	0.065	0.923
Number of reflections	31376
<I/σ(I)>	6.4
Completeness [%]	99.9	99.9	100
Redundancy	10.9	10.6	10.9
CC(1/2)		0.997	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	22% PEG4000, 0.14 M ammonium sulfate