5UEG
RNA primer-template complex with guanosine dinucleotide G(5')pppp(5')G ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.995 |
Spacegroup name | P 3 |
Unit cell lengths | 48.416, 48.416, 81.965 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.21197 |
Rwork | 0.208 |
R-free | 0.30148 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dhc |
RMSD bond length | 0.018 |
RMSD bond angle | 3.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.100 | 0.554 |
Number of reflections | 6425 | |
<I/σ(I)> | 15.14 | 1.26 |
Completeness [%] | 96.6 | 74.8 |
Redundancy | 5.1 | 3.6 |
CC(1/2) | 0.978 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05 M Magnesium chloride, 1.2 M Lithium sulfate, 50 mM MES pH 6.5, 2 mM Cobalt (II) chloride |