5UEE
RNA primer-template complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 46.964, 46.964, 83.103 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.23406 |
| Rwork | 0.232 |
| R-free | 0.27469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.659 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.464 |
| Number of reflections | 8742 | |
| <I/σ(I)> | 35.3 | 1.86 |
| Completeness [%] | 99.4 | 94.4 |
| Redundancy | 9.1 | 4.5 |
| CC(1/2) | 0.979 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05 M Magnesium chloride, 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |
