5UED
RNA primer-template complex with guanosine dinucleotide ligand G(5')pp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-07-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.997 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 43.911, 43.911, 85.406 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.500 |
R-factor | 0.21147 |
Rwork | 0.210 |
R-free | 0.23216 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dhc |
RMSD bond length | 0.025 |
RMSD bond angle | 3.216 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.079 |
Number of reflections | 15113 |
<I/σ(I)> | 28.4 |
Completeness [%] | 95.1 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05 M magnesium chloride, 0.2 M Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350 |