5UEA

Structure of antigen-Fab complex with Histone chaperone ASF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97900
Spacegroup name	P 1
Unit cell lengths	52.561, 81.770, 82.150
Unit cell angles	70.51, 77.28, 81.67

Refinement procedure

Resolution	39.347 - 1.701
R-factor	0.1566
Rwork	0.154
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.852
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.347
High resolution limit [Å]	1.701
Number of reflections	133679
<I/σ(I)>	8.1
Completeness [%]	97.0
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, .2 M Ammonium chloride