5UDZ

Human LIN28A in complex with let-7f-1 microRNA pre-element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	76.429, 76.429, 105.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.520 - 2.000
R-factor	0.1659
Rwork	0.164
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ts2
RMSD bond length	0.011
RMSD bond angle	1.277
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.520	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.091	0.619
Number of reflections	40547
<I/σ(I)>	10.79	1.73
Completeness [%]	99.0	96.96
Redundancy	2.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.1 M Citrate, pH 8.0