5UCV

Crystal Structure of a Ribosome Biogenesis GTP-binding protein (YsxC) from Neisseria gonorrhoeae with bound GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	52.110, 68.440, 126.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.204 - 1.800
R-factor	0.1691
Rwork	0.167
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dhe
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.204	48.204	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.074	0.027	0.560
Rmeas	0.081	0.030	0.621
Total number of observations	259276
Number of reflections	42802	550	3082
<I/σ(I)>	17.88	48.06	2.95
Completeness [%]	99.9	97.9	99.7
Redundancy	6.058	5.22	5.351
CC(1/2)	0.999	0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	NegoA.00252.a.B1.PW37899 at 17.5 mg/ml, incubated with 4 mM MgCl2, 3 mM GTP, protein mixed 1:1 and incubated with an equal volume MCSG1(c4): 25.5% (w/v) PEG-4000, 15% (v/v) glycerol, 0.085 M sodium acetate:HCl, 0.17 M ammonium acetate, cryoprotected with 20% ethylene glycol