5UCS
Class II fructose-1,6-bisphosphate aldolase E149A variant of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.056, 82.826, 91.095 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 29.936 - 1.408 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.936 |
| High resolution limit [Å] | 1.408 |
| Rmerge | 0.078 |
| Number of reflections | 108617 |
| <I/σ(I)> | 8.89 |
| Completeness [%] | 65.9 |
| Redundancy | 3.8 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer |
