5UCG
Structure of the PP2C Phosphatase Domain and a Fragment of the Regulatory Domain of the Cell Fate Determinant SpoIIE from Bacillus Subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 125.622, 125.622, 330.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.054 - 3.906 |
| R-factor | 0.2786 |
| Rwork | 0.276 |
| R-free | 0.32250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t91 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 10.570 | 3.900 |
| Rmerge | 0.102 | 0.031 | 1.448 |
| Rmeas | 0.107 | 0.033 | 1.563 |
| Rpim | 0.031 | 0.009 | 0.563 |
| Total number of observations | 284918 | ||
| Number of reflections | 24917 | ||
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 99.7 | 99.4 | 97.4 |
| Redundancy | 11.4 | 11.2 | 6.8 |
| CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mM LiSO4, 8% PEG8000, 0.05 mM NaF, 6% glycerol |
