5UCB
Structure of antigen-Fab complex with engineered switch residue region.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2015-06-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 |
Unit cell lengths | 45.378, 49.180, 84.707 |
Unit cell angles | 106.29, 95.71, 100.13 |
Refinement procedure
Resolution | 35.589 - 1.521 |
R-factor | 0.1396 |
Rwork | 0.138 |
R-free | 0.16880 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.589 |
High resolution limit [Å] | 1.521 |
Number of reflections | 100518 |
<I/σ(I)> | 32.2 |
Completeness [%] | 95.8 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18% PEG3350, |