5UC7
Crystal structure of BioA / 7,8-diaminopelargonic acid aminotransferase / DAPA synthase from Citrobacter rodentium, PLP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.369, 88.306, 63.274 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.153 - 1.835 |
R-factor | 0.1542 |
Rwork | 0.152 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qj5 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.410 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2481) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.430 | 1.841 |
High resolution limit [Å] | 1.835 | 1.835 |
Rmerge | 0.104 | 0.778 |
Number of reflections | 36804 | |
<I/σ(I)> | 12.5 | 2.1 |
Completeness [%] | 97.8 | 90.6 |
Redundancy | 5.4 | 4.8 |
CC(1/2) | 0.998 | 0.661 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M ammonium tartrate pH 7, 20% (w/v) PEG3350 |