5UBY
Fab structure of anti-HIV-1 gp120 mAb 1A8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979460 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.098, 40.121, 77.195 |
| Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
| Resolution | 44.736 - 2.600 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.740 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 24889 | |
| <I/σ(I)> | 19.87 | 2.16 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.15 | 4.18 |
| CC(1/2) | 0.999 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 18% (w/v) polyethylene glycol (PEG) 8000, 0.1 M HEPES pH 7.5 |
