5UBP
TREX2 M-region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.368, 86.634, 168.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.945 - 2.300 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t5v |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.530 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.945 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 51644 | |
| <I/σ(I)> | 7.5 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 % PEG3350, 0.17 M MgFormate |
