5UBP
TREX2 M-region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.368, 86.634, 168.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.945 - 2.300 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t5v |
RMSD bond length | 0.003 |
RMSD bond angle | 0.530 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.945 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 51644 | |
<I/σ(I)> | 7.5 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.1 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 % PEG3350, 0.17 M MgFormate |