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5UBE

Crystal structure of the N-terminal domain (domain 1) of RctB

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]80
Detector technologyCCD
Collection date2014-07-08
DetectorADSC QUANTUM 315
Wavelength(s)0.979
Spacegroup nameP 1 21 1
Unit cell lengths45.803, 52.127, 63.414
Unit cell angles90.00, 101.61, 90.00
Refinement procedure
Resolution33.326 - 2.000
R-factor0.2313
Rwork0.227
R-free0.26560
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5ubd
RMSD bond length0.006
RMSD bond angle0.757
Data reduction softwareHKL-2000 (v714)
Data scaling softwareHKL-2000 (v714)
Phasing softwarePHASER (1.11_2567-000)
Refinement softwarePHENIX (1.11_2567)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.030
High resolution limit [Å]2.0002.000
Number of reflections16800
<I/σ(I)>1716.6
Completeness [%]84.364.6
Redundancy1.61.4
CC(1/2)0.878
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7277Crystals of RctB domain 1 (1-124) were prepared using the sitting drop vapor diffusion method by mixing 0.1, or 0.2, or 0.4 uL of the protein solution (22.4 mg/ml RctB-2-124 in 20 mM Tris pH 7.4, 50 mM sodium chloride, 5% glycerol, 5 mM 2-mercaptoethanol) and 0.2 uL of reservoir solution (0.24 M Sodium malonate pH 7.0, 20% w/v PEG 3350).

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