5UBC
The structure of the Arabidopsis thaliana Toc75 POTRA domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.368, 50.453, 94.802 |
| Unit cell angles | 90.00, 115.56, 90.00 |
Refinement procedure
| Resolution | 47.207 - 2.862 |
| R-factor | 0.2301 |
| Rwork | 0.224 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uay |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.205 |
| Data reduction software | DENZO (HKL2000) |
| Data scaling software | SCALEPACK (HKL2000) |
| Phasing software | PHASER (PHENIX) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 16654 | |
| <I/σ(I)> | 8.8 | 1.3 |
| Completeness [%] | 89.9 | 93.1 |
| Redundancy | 2.4 | 2.4 |
| CC(1/2) | 0.480 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM HEPES:NaOH, pH7.5, 20% PEG 8000 |
