5UBB
Crystal structure of human alpha N-terminal protein methyltransferase 1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-25 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 45.595, 132.962, 42.292 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.980 - 2.000 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.980 | 45.600 | 2.050 |
High resolution limit [Å] | 2.000 | 8.950 | 2.000 |
Rmerge | 0.086 | 0.019 | 0.700 |
Rmeas | 0.106 | 0.023 | 0.871 |
Rpim | 0.060 | 0.013 | 0.506 |
Total number of observations | 44067 | 453 | 3103 |
Number of reflections | 16827 | ||
<I/σ(I)> | 10.2 | 43.1 | 1.7 |
Completeness [%] | 94.4 | 79.6 | 91.7 |
Redundancy | 2.6 | 2.5 | 2.6 |
CC(1/2) | 0.996 | 0.999 | 0.623 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG3350, 0.2 M sodium acetate |