5UBA
Human RNA Pseudouridylate Synthase Domain Containing 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-16 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97891 |
Spacegroup name | P 43 |
Unit cell lengths | 54.189, 54.189, 105.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.500 - 1.540 |
R-factor | 0.1979 |
Rwork | 0.198 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xpi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.442 |
Data reduction software | DENZO |
Data scaling software | HKL-3000 |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.570 |
High resolution limit [Å] | 1.540 | 4.180 | 1.540 |
Rmerge | 0.054 | 0.028 | 0.840 |
Number of reflections | 44925 | ||
<I/σ(I)> | 6.1 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 11 | 11.4 | 11.2 |
CC(1/2) | 1.000 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 0.2 M CaCl2 |