5UAZ
Crystal structure of the yeast nucleoporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.497, 100.487, 85.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.282 - 1.753 |
| R-factor | 0.1585 |
| Rwork | 0.156 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p3d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.282 | 1.816 |
| High resolution limit [Å] | 1.753 | 1.753 |
| Rmerge | 0.119 | |
| Number of reflections | 18275 | |
| <I/σ(I)> | 21.66 | 2.55 |
| Completeness [%] | 99.8 | 98.72 |
| Redundancy | 13.3 | 9.9 |
| CC(1/2) | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% (wt/vol) PEG 3350 0.2 M Ammonium sulfate |
