5UAY
The structure of the Arabidopsis thaliana Toc75 POTRA domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.289, 54.773, 135.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.739 - 2.500 |
| R-factor | 0.2045 |
| Rwork | 0.199 |
| R-free | 0.25150 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.223 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13243 | |
| <I/σ(I)> | 33.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.5 |
| CC(1/2) | 0.620 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM HEPES:NaOH, pH7.5, 20% PEG 8000 |
