5UAS
Structure of a new family of Polysaccharide lyase PL25-Ulvanlyase bound to -[GlcA(1-4)Rha3S]-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9871 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.620, 71.810, 109.270 |
| Unit cell angles | 90.00, 122.44, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.600 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uam |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.122 |
| Data scaling software | XDS |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.800 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.097 |
| Number of reflections | 143590 |
| <I/σ(I)> | 13.75 |
| Completeness [%] | 95.4 |
| Redundancy | 7.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 15% PEG3500, 0.2M ammonium acetate, 0.1M Bis-Tris pH 5 |
