5UAQ
Escherichia coli RNA polymerase RpoB H526Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9769 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 185.361, 206.280, 308.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.901 - 3.600 |
R-factor | 0.2471 |
Rwork | 0.246 |
R-free | 0.30490 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 134049 | |
<I/σ(I)> | 12 | |
Completeness [%] | 97.3 | |
Redundancy | 6.2 | |
CC(1/2) | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT |