5UAQ

Escherichia coli RNA polymerase RpoB H526Y mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9769
Spacegroup name	P 21 21 21
Unit cell lengths	185.361, 206.280, 308.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.901 - 3.600
R-factor	0.2471
Rwork	0.246
R-free	0.30490
RMSD bond length	0.022
RMSD bond angle	2.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.660
High resolution limit [Å]	3.600	3.600
Number of reflections	134049
<I/σ(I)>	12
Completeness [%]	97.3
Redundancy	6.2
CC(1/2)		0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT