5UAI
Crystal structure of Methionyl-tRNA formyltransferase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 113.920, 109.060, 109.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.084 - 2.750 |
R-factor | 0.1709 |
Rwork | 0.167 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fmt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.084 | 49.084 | 2.820 |
High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
Rmerge | 0.105 | 0.028 | 0.531 |
Number of reflections | 36266 | ||
<I/σ(I)> | 18.88 | 36.43 | 4.36 |
Completeness [%] | 99.9 | 96 | 100 |
Redundancy | 7.402 | 5.432 | 7.546 |
CC(1/2) | 0.998 | 0.999 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 20.7 mg/mL PsaeA.17389.a.B1.PW37587 crystallized in sitting drop vapor diffusion with Rigaku MCSG1 B5: 0.2 M MgCl2; 0.1 M Tris pH 8.5; 25% PEG3350, cryo: 20% EG. tray 259534b5, puck rzw4-7 |