5UAH

Escherichia coli RNA polymerase and Rifampin complex, RpoB D516V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9779
Spacegroup name	P 21 21 21
Unit cell lengths	187.814, 206.668, 310.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.740 - 4.100
R-factor	0.2438
Rwork	0.243
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yg2
RMSD bond length	0.008
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.170
High resolution limit [Å]	4.100
Number of reflections	96868
<I/σ(I)>	13.9
Completeness [%]	99.8
Redundancy	7.4
CC(1/2)		0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT