5UAE
Crystal structure of the coiled-coil domain from Listeria Innocua Phage Integrase (Trigonal Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.432, 75.432, 103.061 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.716 - 2.750 |
| R-factor | 0.2213 |
| Rwork | 0.216 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kis |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.585 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.716 | 2.848 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.052 | 0.623 |
| Number of reflections | 69598 | |
| <I/σ(I)> | 15.3 | 1.8 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294.15 | 0.8-1.2 M Na Citrate |
