5UA1
Mycobacterium tuberculosis KstR in complex with a 18-bp DNA operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.423, 79.280, 116.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.853 - 2.900 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cxg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.519 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.155 | 1.045 |
| Number of reflections | 24284 | |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.993 | 0.470 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 15% PEG 3350, 9% MPD, 0.2 M Li2SO4, 0.1 M imidazole-HCl |
