5U9L
Crystal structure of the intermembrane space region of the plastid division protein PARC6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.533, 124.546, 130.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.910 - 2.516 |
R-factor | 0.1851 |
Rwork | 0.181 |
R-free | 0.25940 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.600 |
High resolution limit [Å] | 2.500 | 5.200 | 2.500 |
Rmerge | 0.139 | 0.095 | 0.907 |
Number of reflections | 12543 | ||
<I/σ(I)> | 6.2 | ||
Completeness [%] | 99.5 | 99.1 | 98.4 |
Redundancy | 8.3 | 8.9 | 7 |
CC(1/2) | 0.996 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 18% polyethylene glycol (PEG) 4000, 0.1 M Magnesium acetate, 0.1 M Na-MES (pH 6.5), 0.05 M Potassium nitrate and 5% glycerol |