5U93
Structure of the Regulatory Domain of the AraC Family Transcriptional Activator RhaR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 100.793, 100.793, 95.696 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.863 - 1.999 |
R-factor | 0.1724 |
Rwork | 0.171 |
R-free | 0.20300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.084 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 1.999 | 2.050 |
Rmerge | 0.034 | 0.203 |
Number of reflections | 33840 | |
<I/σ(I)> | 33.128 | 3.96 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 9.2 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES pH 6.0, 50 mM Ca(OAc)2, 12 % PEG 3350 |