5U8K
RitR mutant - C128S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.128, 60.079, 53.324 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 40.329 - 1.690 |
| R-factor | 0.1466 |
| Rwork | 0.146 |
| R-free | 0.16860 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.330 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 91682 | |
| <I/σ(I)> | 16.6 | 8.1 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 5.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 0.1 M ammonium acetate, 25% PEG 3350 |
