5U8J

Crystal structure of a protein of unknown function ECL_02571 involved in membrane biogenesis from Enterobacter cloacae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-12-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	87.170, 53.890, 90.000
Unit cell angles	90.00, 100.04, 90.00

Refinement procedure

Resolution	29.564 - 2.520
R-factor	0.2157
Rwork	0.213
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bz6
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.560
High resolution limit [Å]	2.520	2.520
Rmerge	0.114	0.922
Number of reflections	28186
<I/σ(I)>	19.84	2.17
Completeness [%]	98.2	94.6
Redundancy	5	4.8
CC(1/2)		0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	298	20% PEG3350, 0.2 M potassium dihydrogen phosphate, 1% sucrose, 0.1 M phosphate citrate pH 4.2