5U8G
DNA Polymerase Beta crystallized in PEG 400
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 104 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.104, 78.659, 54.721 |
Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
Resolution | 13.680 - 2.166 |
R-factor | 0.1839 |
Rwork | 0.179 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
RMSD bond length | 0.002 |
RMSD bond angle | 0.495 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 14.000 | 14.000 | 2.250 |
High resolution limit [Å] | 2.166 | 4.620 | 2.166 |
Rmerge | 0.112 | 0.062 | 0.622 |
Number of reflections | 45604 | ||
<I/σ(I)> | 13.7 | ||
Completeness [%] | 99.8 | 100 | 98.1 |
Redundancy | 11.6 | 14.1 | 4.3 |
CC(1/2) | 0.999 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 24% PEG 400, 30 mM potassium sodium tartrate, 1% 1,4-dioxane |