5U85

Murine saposin-D (SapD), open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-30
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	41.650, 43.163, 88.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.822 - 1.650
R-factor	0.1672
Rwork	0.166
R-free	0.18570
Structure solution method	AB INITIO PHASING
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	ACORN
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	19863
<I/σ(I)>	33.2
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	sodium citrate PEG 4000 isopropanol