5U6Z

Crystal Structure of Xenopus laevis Apex2 C-terminal Znf-GRF Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1.28257
Spacegroup name	I 4
Unit cell lengths	90.332, 90.332, 23.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.166 - 2.600
R-factor	0.2
Rwork	0.195
R-free	0.24460
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.460
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.074	0.058	0.314
Number of reflections	5570
<I/σ(I)>	8
Completeness [%]	97.9	99.3	84.4
Redundancy	3.7	3.8	3.2
CC(1/2)		0.994	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 M (NH4)2SO4, 0.1 M Bicine pH 9.0