5U6M

Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and salicylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	65.694, 87.681, 164.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.841 - 2.568
R-factor	0.1914
Rwork	0.188
R-free	0.25990
RMSD bond length	0.011
RMSD bond angle	1.213
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	30702
<I/σ(I)>	0.086	1.7
Completeness [%]	99.3	98
Redundancy	6.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	22-26 % (w/v) PEG3350, 0.2 M ammonium acetate, 0.1 M MES