5U5G
Psf3 in complex with NADP+ and 2-OPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.076, 87.369, 164.011 |
| Unit cell angles | 90.00, 92.04, 90.00 |
Refinement procedure
| Resolution | 46.324 - 2.048 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.581 | |
| Number of reflections | 78862 | |
| <I/σ(I)> | 9.6 | 2 |
| Completeness [%] | 96.6 | 96.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 12 mg/mL Psf3, 43% v/v polypropylene glycol P400, 0.1 M Bis-Tris Methane pH 6.5 |
