5U51
Structure of Francisella tularensis heterodimeric SspA (MglA-SspA) in complex with ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.620, 111.560, 141.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.610 - 2.800 |
R-factor | 0.2151 |
Rwork | 0.212 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pur 1yy7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.526 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.610 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.874 | |
Rpim | 0.404 | |
Number of reflections | 23247 | |
<I/σ(I)> | 7.8 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | TRIS pH 8.5, PEG3350, ppGpp, magnesium chloride |